| ORIGINAL ARTICLE |
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| Year : 2020 | Volume
: 9
| Issue : 3 | Page : 152-156 |
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Antibacterial activity of ethanol extract of the leaves of Rosmarinus officinalis L. at different concentrations versus Streptococcus mutans: An In vitro comparative study
Edgar Janampa1, Hugo Perez1, Franco Mauricio1, Daniel Alvítez-Temoche1, Cesar Mauricio-Vilchez1, Walter Gallo2, Frank Mayta-Tovalino3
1 Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Lima, Peru
2 Social Responsibility Center, Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, Peru
3 Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal; Postgraduate Department, Faculty of Health Sciences, Universidad Científica del Sur, Lima, Peru
Correspondence Address:
Dr. Frank Mayta-Tovalino
Postgraduate Department, Faculty of Health Sciences, Universidad Cientifica del Sur, Lima
Peru
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/ejgd.ejgd_148_20
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Aim: The study aimed to compare the in vitro antibacterial activity of an ethanol extract of Rosmarinus officinalis “romero” (EERO) at different concentrations with Streptococcus mutans ATCC 25175. Materials and Methods: We worked with three concentrations of 25%, 50%, and 75% of the EERO and compared these with the positive control chlorhexidine 0.12% and distilled water as a negative control. Seeding was performed in Müller-Hinton agar medium. The inhibitory effectiveness of EERO was determined using the disc diffusion method with the experimental solutions. The seeded and inoculated plates were incubated at 37°C, for 24 and 48 h. The reading was carried out according to the Kirby-Bauer method by measuring the diameter (mm) of the inhibition halo formed by the bacteria using a Vernier caliper and recorded on a data collection sheet. Results: In the 24-h group, chlorhexidine 0.12% showed the highest inhibition halos with a mean of 12.8 ± 0.8 mm, followed by EERO 75% with a similar mean of 12.1 ± 0.6 mm. Similarly, at 48 h, chlorhexidine 0.12% had the highest antibacterial activity of 13.0 ± 0.7 mm followed by EERO 75% with 12.2 ± 0.5 mm. Conclusions: The EERO showed inhibitory effectiveness against S. mutans, with the concentration of 75 mg/ml showing similar results, albeit not statistically significantly different, to those of chlorhexidine.
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