Home Current issue Instructions
About us Archives Login 
Editorial board Search articles Contact us
Home Print this page Email this page Users Online: 197
Year : 2020  |  Volume : 9  |  Issue : 3  |  Page : 157-162

Effects of the incorporation of alpha-tocopherol as antioxidant on biological and physicochemical properties of calcium hydroxide associated with bioactive vehicle

1 Department of Restorative Dentistry, School of Dentistry Araraquara, Sao Paulo State University – UNESP, Araçatuba, Sao Paulo, Brazil
2 Department of Basic Sciences, Araçatuba Dental School, Sao Paulo State University – UNESP, Araçatuba, Sao Paulo, Brazil

Correspondence Address:
Prof. Joissi Ferrari Zaniboni
Humaita Street, 1740 - Center, Araraquara, Sao Paulo
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ejgd.ejgd_134_20

Rights and Permissions

Aim: The aim of this study to evaluate the effects of 10% alpha-tocopherol (10AT) or 20% alpha-tocopherol (20AT) incorporation on biological compatibility, pH, and calcium release of calcium hydroxide (CH) paste associated with 2% chlorhexidine digluconate (CHX). Materials and Methods: Four groups were evaluated: CH, CH-CHX, CH-CHX-10AT, and CH-CHX-20AT. For biological compatibility test, polyethylene tubes containing several pastes were implanted in Wistar rats' subcutaneous tissue (n = 28). After 48 h and 7, 14, and 21 days postimplantation, the specimens were removed and subjected to histologic and histomorphometric analysis. The number of inflammatory cells was evaluated. For pH and calcium release analysis, the pastes were placed individually (n = 10) in plastic tubes and immersed in deionized water. The calcium release and pH changes were evaluated in 24 and 48 h and 7, 14, and 21 days. All data were submitted to Kruskal–Wallis test (α = 0.05). Results: Concerning biological compatibility, all materials shown a similar decidual response (P > 0.05). In the first hours, there was as increase in the number of inflammatory cells, inducing an expressive inflammatory response. After 14 days, inflammation reaction decreased and collagen fiber was organized for the tested pastes (P = 0.05). The pH analysis of the groups maintained the same relationship during the different periods evaluated: the CH and CH-CHX groups showed higher values and were similar to each other (P > 0.05), followed by the CH-CHX-10AT and CH-CHX-20 AT groups. Regarding the amount of calcium ions, in the initial (24 hours) and final (21 days) periods, the groups did not present differences between them (P > 0.05). Conclusion: The 10AT or 20AT, as an antioxidant agent, incorporation to CH and 2% CHX paste negatively affected biological and physicochemical properties.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded75    
    Comments [Add]    

Recommend this journal