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Year : 2020  |  Volume : 9  |  Issue : 3  |  Page : 152-156

Antibacterial activity of ethanol extract of the leaves of Rosmarinus officinalis L. at different concentrations versus Streptococcus mutans: An In vitro comparative study

1 Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal, Lima, Peru
2 Social Responsibility Center, Faculty of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, Peru
3 Academic Department, Faculty of Dentistry, Universidad Nacional Federico Villarreal; Postgraduate Department, Faculty of Health Sciences, Universidad Científica del Sur, Lima, Peru

Correspondence Address:
Dr. Frank Mayta-Tovalino
Postgraduate Department, Faculty of Health Sciences, Universidad Cientifica del Sur, Lima
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ejgd.ejgd_148_20

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Aim: The study aimed to compare the in vitro antibacterial activity of an ethanol extract of Rosmarinus officinalis “romero” (EERO) at different concentrations with Streptococcus mutans ATCC 25175. Materials and Methods: We worked with three concentrations of 25%, 50%, and 75% of the EERO and compared these with the positive control chlorhexidine 0.12% and distilled water as a negative control. Seeding was performed in Müller–Hinton agar medium. The inhibitory effectiveness of EERO was determined using the disc diffusion method with the experimental solutions. The seeded and inoculated plates were incubated at 37°C, for 24 and 48 h. The reading was carried out according to the Kirby–Bauer method by measuring the diameter (mm) of the inhibition halo formed by the bacteria using a Vernier caliper and recorded on a data collection sheet. Results: In the 24-h group, chlorhexidine 0.12% showed the highest inhibition halos with a mean of 12.8 ± 0.8 mm, followed by EERO 75% with a similar mean of 12.1 ± 0.6 mm. Similarly, at 48 h, chlorhexidine 0.12% had the highest antibacterial activity of 13.0 ± 0.7 mm followed by EERO 75% with 12.2 ± 0.5 mm. Conclusions: The EERO showed inhibitory effectiveness against S. mutans, with the concentration of 75 mg/ml showing similar results, albeit not statistically significantly different, to those of chlorhexidine.

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