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Year : 2019  |  Volume : 8  |  Issue : 3  |  Page : 71-75

Influence of blood contamination and decontamination procedures on bond strength of a two-step etch and rinse adhesive system

1 Graduate Program in Dentistry, Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil
2 Department of Restorative Dentistry, Metropolitan Faculty of Grande Fortaleza, Fortaleza, Ceará, Brazil
3 Graduate Program in Dentistry, Department of Restorative Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil; Dental Materials Laboratory, Academic Area of Dentistry, Autonomous University of Hidalgo State, Pachuca, Hidalgo, México

Correspondence Address:
Prof. Rafael Guerra Lund
Department of Restorative Dentistry, Pelotas Dental School, Federal University of Pelotas, Gonçalves Chaves Street N. 457, Rm. 503, Centro District, 96015-560 Pelotas, Rio Grande do Sul
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/ejgd.ejgd_57_19

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Purpose: This study evaluated the effect of blood contamination and decontamination procedures performed at different stages of bonding, on the microtensile bond strength (μTBS) of an etch-and-rinse adhesive to dentin. Materials and Methods: Standardized cylindrical cavities were prepared in bovine incisors and were randomly divided into five groups, each one was treated using these experimental conditions – Control: etching-rinsing/bonding without blood contamination; Group 1: etching-rising/blood contamination/decontamination/bonding; Group 2: etching-rising/bonding/blood contamination/decontamination; Group 3: etching-rising/bonding/blood contamination/decontamination/etching-rising/bonding; and Group 4: etching-rising/blood contamination/decontamination/etching-rising/bonding. Specimens were prepared for μTBS and were evaluated both immediately and after 6 months of storage in distilled water. Results: Blood contamination significantly reduced the μTBS (P < 0.001). Groups 2 and 3 showed the lowest μTBS values, both for 24 h and 6 month aging. Conclusions: Blood contamination tested at any of the different stages of the bonding procedure showed a negative effect on the μTBS. Recovering adhesion of blood-contaminated dentin did not depend only on cleaning with distilled water.

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