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Year : 2013  |  Volume : 2  |  Issue : 2  |  Page : 130-133

Evaluation of microflora of root carious lesions in different age groups: A microbiological study

1 Department of Conservative Dentistry and Endodontics, A. B. Shetty Memorial Institute of Dental Sciences, Deralakatte, Mangalore, Karnata, India
2 Department of Microbiology, K.S. Hegde Medical Academy, Nitte University, Deralakatte, Mangalore, Karnataka, India

Correspondence Address:
Ashok Aparna
Department of Conservative Dentistry and Endodontics, A.B. Shetty Memorial Institute of Dental Sciences, Nitte University, Deralakatte, Mangalore - 575 018, Karnataka
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2278-9626.112310

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Aim: To evaluate the prevalence of mutans streptococci, lactobacilli and Actinomyces species in root carious lesions in middle-aged and older adults of South Indian population and to comparatively evaluate the microbial count in two different age groups. Materials and Methods: Specimens of root carious lesions were collected from 60 adults for bacteriological studies. Amongst which 28 subjects belonged to middle age group (35-44 years) and 32 subjects to older age group (55-75 years). Standard procedures of culture, isolation and identification of aerobic and anaerobic bacteria were adopted in the study. Results: The analysis of results was performed using Chi-square test. SPSS 20 has been used to analyze the data. Among the isolated microorganisms, streptococci (56.7%) and lactobacilli (28.3%) were more prevalent in root caries lesions compared to actinomyces species (15.0%) and most frequently occurred in older adults. There is also a statistically significant association between the age group and the presence of lactobacillus (P=0.024). Conclusions: Aerobic Gram-positive cocci (Streptococcus mutans, lactobacillus) as well as anaerobic bacteria (Actinomyces spp.), occurred most frequently in root carious lesions in older adults as compared to middle-aged population.

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